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By Research Topic
Musculoskeletal Biology and Tissue Engineering
Computer Vision-Based Tracking of Musculoskeletal Cells
Musculoskeletal Biology and Tissue Engineering
- Zhang, X., Li, K., Wang, C.Y., Rao, Y., Tuan, R.S., Wang, D., Ker, D.F.E.¶ (Jul 2024) ‘Facile and Rapid Fabrication of a Novel 3D-Printable, Visible Light-Crosslinkable and Bioactive Polythiourethane for Large-to-Massive Rotator Cuff Tendon Repair’ Bioactive Materials 37, pg. 439. https://doi.org/10.1016/j.bioactmat.2024.03.036. ¶Corresponding author. Notable for developing a 3D-printable visible light-crosslinkable resin that can be prepared in 30 mins. This resin can generate mechanically strong and tendon-like biomaterial that withstands 10,000 cycles of loading without failure and can deliver pro-regenerative bioactive factors. This bioactive material biomechanically restored a large-to-massive (0.3-cm segmental defect with 1-cm gap) rabbit rotator cuff injury model to uninjured levels with at least 1-cm of tendon regeneration.
- Huang, S., Rao, Y., Zhou, M., Blocki, A.M., Ker, D.F.E., Tuan, R.S., Wang, D, (Jun 2024) ‘Engineering an extracellular matrix-functionalized, load-bearing tendon substitute for effective repair of large-to-massive tendon tears’ Bioactive Materials 36, pg. 221. https://doi.org/10.1016/j.bioactmat.2024.02.032. Notable for employing ex vivo, in vitro, and in vivo methods for fabricating a mechanically strong and bioactive material to address large tendon defects, which was assessed in two (rat and rabbit) rotator cuff injury models. and bioactive material to address large tendon defects, which was assessed in two (rat and rabbit) rotator cuff injury models.
- Wang, C.Y., Zhang, X., Wang, D, Yung, P.S.H., Tuan, R.S., Ker, D.F.E. (Mar 2024) ‘Optimized design of an enthesis-mimicking suture anchor-tendon hybrid graft for mechanically robust bone-tendon repair’ Acta Biomaterialia 176, pg. 277. https://doi.org/10.1016/j.actbio.2024.01.011. *Notable for employing in silico, ex vivo, and in vivo methods to optimize design of bone-tendon biomaterials to address a grand challenge in musculoskeletal tissue engineering: Reducing stress concentrations to facilitate robust attachment of soft-to-hard tissues.
- Huang, S., Rao, Y., Ju, A.L., Ker, D.F.E., Blocki, A.M., Wang, D, Tuan, R.S. (Mar 2024) ‘Non-collagenous proteins, rather than the collagens, are key biochemical factors that mediate tenogenic bioactivity of tendon extracellular matrix’ Acta Biomaterialia 176, pg. 99. https://doi.org/10.1016/j.actbio.2023.12.032.
- Kwan, K.Y.C., Ng, K.W.K., Rao, Y., Zhu, C., Qi, S., Tuan, R.S., Ker, D.F.E., Wang, D. (Oct 2023) ‘Biology of Tendon Aging: Mechanisms, Function, and Advanced Cell Therapeutics’ International Journal of Molecular Sciences 24, 20, pg. 15183 https://doi.org/10.3390/ijms242015183
- Li, K., Zhang, X., Wang, D., Ker, D.F.E. (Mar 2023) ‘Development of a Rabbit Chronic-Like Rotator Cuff Injury Model for Study of Fibrosis and Muscular Fatty Degeneration’ Journal of Visualized Experiments Mar 31, (193). https://doi.org/10.3791/64828.
- Li, K., Zhang, X., Wang, D., Tuan, R.S., Ker, D.F.E. (Mar 2023) ‘Synergistic effects of growth factor-based serum-free medium and tendon-like substrate topography on tenogenesis of mesenchymal stem cells’ Biomaterials Advances 146, pg. 213316. https://doi.org/10.1016/j.bioadv.2023.213316. *Notable for developing a serum-free medium formulation that supports tenogenic proliferation and differentiation in vitro as well as demonstrating synergistic interactions between growth factor and substrate topography on mesenchymal stem cell tenogenesis in vitro.
- Wang, D*., Zhang, X., Ng, K.W., Rao, Y., Wang, C.Y., Gharaibeh, B., Lin, S., Abrams, G., Safran, M., Cheung, E., Campbell, P., Weiss, L., Ker, D.F.E.* ¶, Yang, Y.P.¶ (Dec 2022) ‘Growth and Differentiation Factor-7 Immobilized, Mechanically Strong Quadrol-Hexamethylene Diisocyanate-Methacrylic Anhydride Polyurethane Polymer for Tendon Repair and Regeneration’ Acta Biomaterialia 154, pg. 108. *Co-first authors. ¶Co-corresponding authors. https://doi.org/10.1016/j.actbio.2022.10.029. *Notable for interfacing for methodology to augment synthetic biomaterials with bioactivity via growth factor immobilization for tendon regeneration.
- Shen, Y., Liu, H., Zhao, B., Pooh, H.L.E., Qi, S., Ker, D.F.E., Billiar, T.R., Cooper, G.M., Xu, Y., Wang, D. (Dec 2022) ‘Short-term Administration of the HIV Protease Inhibitor Saquinavir Improves Skull Bone Healing via Enhanced Osteoclastogenesis’ Plastic and Reconstructive Surgery 150, pg. 1264e. https://doi.org/10.1097/PRS.0000000000009734.
- Cheng, C.T., Wang, D., Wang, H., Ker, D.F.E. (Nov 2022) ‘Application of neural networks on in vitro-generated Raman spectra for label-free, ex vivo skeletal muscle detection’ Measurement 204, pg. 112172. https://doi.org/10.1016/j.measurement.2022.112172. *Notable for establishing similarity in complex Raman spectra between in vitro cultured cells and ex vivo tissue, and using of transfer learning for label-free detection of skeletal muscle.
- Zhang, X., Wang, D., Wang, Z., Ling, S.K., Yung, P.S., Tuan, R.S., Ker, D.F.E. (Sep 2022) ‘Clinical Perspectives for Repairing Rotator Cuff Injuries with Multi-Tissue Regenerative Approaches’ Journal of Orthopaedic Translation 36, pg. 91. https://www.sciencedirect.com/science/article/pii/S2214031X22000547?via%3Dihub.
- Rao, Y., Zhu, C., Suen, H. C., Huang, S., Liao, J., Ker, D.F.E., Tuan, R. S., and Wang, D. (Jul 2022) ‘Tenogenic induction of human adipose-derived stem cells by soluble tendon extracellular matrix: composition and transcriptomic analyses’ Stem Cell Research & Therapy 13, pg. 380. https://stemcellres.biomedcentral.com/articles/10.1186/s13287-022-03038-0.
- Zhang, X., Wang, D., Mak, K.K., Tuan, R.S., Ker, D.F.E. (Aug 2021) ‘Engineering Musculoskeletal Grafts for Multi-Tissue Unit Repair: Lessons from Developmental Biology and Wound Healing’ Frontiers in Physiology 12, pg. 1287. https://www.frontiersin.org/articles/10.3389/fphys.2021.691954/abstract.
- Wang, D., Zhang, X., Huang, S., Liu, Y., Fu, B.S., Mak, K.K., Blocki, A.M., Yung, P.S., Tuan, R.S., Ker, D.F.E. (May 2021) ‘Engineering Multi-Tissue Units for Regenerative Medicine: Bone-Tendon-Muscle Units of the Rotator Cuff’ Biomaterials 272, pg. 120789. https://www.sciencedirect.com/science/article/pii/S0142961221001459?via%3Dihub.
- Wang, D.*, Ker, D.F.E.*¶, Ng, K.W., Li, K., Gharaibeh, B., Safran, M., Cheung, E., Campbell, P.G., Weiss, L.E., Yang, Y.P.¶ (Mar 2021) ‘Combinatorial Mechanical-Gradation and Growth Factor-Biopatterning Strategy to Spatially Control Bone-Tendon-like Cell Differentiation and Tissue Formation’ NPG Asia Materials 13, pg. 26. *Co-first authors. ¶Co-corresponding authors. https://www.nature.com/articles/s41427-021-00294-z. *Notable for studying the interaction effect of biopatterned growth factors with substrate stiffness.
- Assunção, M., Yiu, C.H.K., Wan, H.Y., Wang, D., Ker, D.F.E., Tuan, R.S., Blocki, A. (Mar 2021) ‘Hyaluronic acid drives mesenchymal stromal cell-derived extracellular matrix assembly by promoting fibronectin fibrillogenesis’ Journal of Materials Chemistry Part B 9, pg. 7205. https://pubs.rsc.org/en/content/articlelanding/2021/TB/D1TB00268F#!divAbstract.
- Shanjani, Y., Siebert, S.M., Ker, D.F.E., Mercado-Pagan, A.E., and Yang, Y.P. (Jun 2020) ‘Acoustic Patterning of Growth Factor for 3D Tissue Engineering’ Tissue Engineering Part A 26, 11-12, pg. 602. https://www.liebertpub.com/doi/full/10.1089/ten.TEA.2019.0271.
- Wang, D., Pun, C.C.M., Huang, S. Tang, T.C.M., Ho, K.K.W., Rothrauff, B.B., Yung, P.S.H., Blocki, A.M., Ker, D.F.E., and Tuan, R.S. (Apr 2020) ‘Tendon-derived Extracellular Matrix Induces Mesenchymal Stem Cell Tenogenesis via an Integrin/Transforming Growth Factor-β Cross-talk-Mediated Mechanism’ Federation of American Societies for Experimental Biology Journal 34, 6, pg. 8172. https://faseb.onlinelibrary.wiley.com/doi/abs/10.1096/fj.201902377RR.
- Wang, D., Gilbert, J.R., Zhang, X., Zhao, B., Ker, D.F.E., Cooper, G.M (Jan 2020) ‘Calvarial versus Long Bone Implications for Tailoring Skeletal Tissue Engineering’ Tissue Engineering Part B 26, 1, pg. 46. https://www.liebertpub.com/doi/full/10.1089/ten.TEB.2018.0353.
- Ker, D.F.E. and Yang, Y.P. (Jun 2019) ‘Ruminants: Evolutionary Past and Future Impact' Science (Perspective) 364, 6446, pg. 1130. https://science.sciencemag.org/content/364/6446/1130.
- Ker, D.F.E., Wang, D., Sharma, S., Zhang, B., Passarelli, B., Neff, N., Li, C., Maloney, W., Quake S. and Yang, Y.P. (Oct 2018) ‘Identifying Deer Antler uhrf1 Proliferation and s100a10 Mineralization Genes Using Comparative RNA-seq’ Stem Cell Research & Therapy 9, 1, pg. 292. https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/30376879/. *Notable for development of a unique model to study rapid bone regeneration.
- Ker, D.F.E.*¶, Wang, D.*, Behn, A.W., Wang, E.T.H., Zhang, X., Zhou, B.Y., Mercado-Pagan, A.E., Kim, S., Kleimeyer, J., Gharaibeh, B., Shanjani, Y., Nelson, D., Safran, M., Cheung E., Campbell, P., Yang, Y.P.¶ (May 2018) ‘Functionally-Graded, Bone- and Tendon-Like Polyurethane for Rotator Cuff Repair’ Advanced Functional Materials 28, 20, pg. 1707107. *Co-first authors. ¶Co-corresponding authors. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5959293/. *Notable for development of a patented mechanically robust photocrosslinkable biomaterial that achieves bone- and tendon-like mechanical properties
- Shanjani, Y., Kang, Y., Zarnescu, L., Bowden, A.K., Koh, J., Ker, D.F.E. and Yang, Y.P. (Jan 2017) ‘Endothelial Pattern Formation in Hybrid Constructs of Additive Manufactured Porous Rigid Scaffolds and Cell-laden Hydrogels for Orthopedic Applications’ Journal of the Mechanical Behavior of Biomedical Materials 65, pg. 356. https://www.sciencedirect.com/science/article/pii/S175161611630306X.
- Ker, D.F.E., Wang, E.T.H., Sharma, R. and Yang, Y.P. (Sep 2015) ‘Development of mRuby2-transfected C3H10T1/2 Fibroblasts for Musculoskeletal Tissue Engineering’ PLoS ONE 10, 1, pg. e0139054. https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0139054. *A unique fluorescently labelled progenitor cell line that is available from American Type Culture Collection (https://www.atcc.org/products/crl-3268)
- Mercado-Pagan, Ker, D.F.E., and Yang, Y. (Jun 2014) ‘Hemocompatibility Evaluation of Elastomeric Hollow Fiber Membranes as Vascular Grafts’ Journal of Biomaterials Applications 29, 4, pg. 557. https://journals.sagepub.com/doi/10.1177/0885328214537541.
- Mercado-Pagan, A.E., Kang, Y., Ker, D.F.E., Park, S., Yao, J., Bishop, J., and Yang, Y. (Oct 2013) ‘Synthesis and Characterization of Novel Elastomeric Poly(D,L-Lactide Urethane) Maleate Composites for Bone Tissue Engineering’ European Polymer Journal 49, 10, pg. 3337. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4012890/.
- Smith, D.M., Cray, J.J.Jr, Weiss, L.E., Ker, D.F.E., Shakir, S., Rottgers, S.A., Losee, J.E., Campbell, P.G. and Cooper, G.M. (Oct 2012) ‘Precise Control of Osteogenesis for Craniofacial Defect Repair: The Role of Direct Osteoprogenitor Contact in BMP-2-based Bioprinting’ Annals of Plastic Surgery 69, 4, pg. 485. https://journals.lww.com/annalsplasticsurgery/fulltext/2012/10000/Precise_Control_of_Osteogenesis_for_Craniofacial.38.aspx.
- Gao, Y., Smith, E., Ker, D.F.E., Campbell, P.G., Cheng, E., Zou, S., Lin, S., Wang, L., Halene, S. and Krause, D.S. (Mar 2012) ‘Role of RhoA Specific Guanine Exchange Factors in Regulation of Endomitosis in Megakaryocytes’ Developmental Cell 22, 3, pg. 573. https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22387001/.
- Ker, D.F.E., Nain, A., Weiss, L.E., Wang, J., Suhan, J., Amon, C. and Campbell, P.G. (Nov 2011) ‘Bioprinting of Growth Factors onto Aligned Sub-micron Fibrous Scaffolds for Simultaneous Control of Cell Differentiation and Alignment’ Biomaterials 32, 32, pg. 8097. https://www.sciencedirect.com/science/article/pii/S0142961211007964. *Notable for being the first demonstration of patterning a primitive muscle-tendon-bone unit on polyurethane nanofibers.
- Ker, D.F.E., Chu, B., Phillippi, J.A., Gharaibeh, B., Huard, J., Weiss, L.E., Campbell, P.G. (May 2011) ‘Engineering Spatial Control of Multiple Differentiation Fates Within a Stem Cell Population’ Biomaterials 32, 13, pg. 3413. https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21316755/. *Notable for being the first demonstration of patterning a primitive muscle-tendon-bone unit.
Computer Vision-Based Tracking of Musculoskeletal Cells
- Nishimura, K., Wang, C., Wantanabe, K., Ker, D.F.E., Bise, R. (Oct 2021) ‘Weakly Supervised Cell Instance Segmentation under Various Conditions’ Medical Image Analysis 73, pg. 102182. https://www.sciencedirect.com/science/article/pii/S1361841521002280.
- Nishimura K., Hayashida J., Chenyang W., Ker, D.F.E., and Bise, R. (2020) “Weakly-Supervised Cell Tracking via Backward-and-Forward Propagation” (ECCV’20) 16th European Conference on Computer Vision (presented 23rd Aug 2020 - 28th Aug 2020, Glasgow, United Kingdom).
- Nishimura K., Ker, D.F.E., and Bise, R. (2019) “Cell Region Recognition in Microscopic Images Using Weakly Supervised Learning” (MIRU’19) 22nd Symposium on Image Recognition and Understanding (presented 29th Jul 2019 – 1st Aug 2019, Osaka, Japan).
- Nishimura K., Ker, D.F.E., and Bise, R. (2019) “Weakly Supervised Cell Segmentation in Dense By Propagating from Detection Map” (MICCAI’19) Medical Image Computing and Computer Assisted Intervention (presented 13th Oct 2019 – 17th Oct 2019, Shenzhen, China).
- Ker, D.F.E., Eom, S., Sanami, S., Bise, R., Pascale, C., Yin, Z., Huh, S., Osuna-Highley, E., Junkers, S.N., Helfrich, C.J., Liang, P.W., Pan, J., Jeong, S., Kang, S.S., Liu, J., Nicholson, R., Sandbothe, M.F., Van, P.T., Liu, A., Chen, M., Kanade, T., Weiss, L.E. and Campbell P.G. (Nov 2018) ‘Phase Contrast Time-lapse Microscopy Datasets with Automated and Manual Cell Tracking Annotations’ Scientific Data 5, pg. 180237. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6233481/. *Notable for being the largest manually curated and computer-tracked phase contrast microscopy dataset of cells in the world.
- Li, M., Li, H, Ker, D.F.E., Su, H. and Yin, Z. (Oct 2015) ‘Cell-Sensitive Phase Contrast Microscopy Imaging by Multiple Exposures’ Medical Image Analysis 25, 1, pg. 111. https://www.sciencedirect.com/science/article/pii/S1361841515000614.
- Li, H., Li, M., Ker, D.F.E., Su, H. and Yin, Z. (2014) “Cell-Sensitive Microscopy Imaging for Cell Image Segmentation” (MICCAI’14) Medical Image Computing and Computer Assisted Intervention 17(Pt 1), pg. 41-48.
- Huh, S., Ker, D.F.E., Su, H., Kanade, T. (2012) “Apoptosis Detection for Adherent Cell Populations in Time-Lapse Phase-Contrast Microscopy Images” (MICCAI'12) In Proc. International Conference on Medical Image Computing and Computer Assisted Intervention 15(Pt 1), pg.331-339.
- Huh, S., Eom, S., Ker, D.F.E., Weiss, L.E and Kanade, T. (2012) “Mitosis Detection of Hematopoietic Stem Cell Populations in Time-Lapse Phase-Contrast Microscope Images.” (ISBI'12) In Proceedings of IEEE International Symposium on Biomedical Imaging pg. 390-393.
- Ker, D.F.E., Weiss, L.E., Junkers, S.N., Chen, M., Yin, Z., Sandboth, M.F., Huh, S., Eom, S., Bise, R., Osuna-Highley, E., Kanade, T. and Campbell, P.G. (Nov 2011) ‘An Engineered Approach to Stem Cell Culture: Automating the Decision Process for Real-Time Adaptive Subculture of Stem Cells’ PLoS ONE 6, 11, pg. e27672. https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0027672 *Notable for development of an online decision-making system that has similar performance as a skilled human operator for culturing cells.
- Eom, S., Huh, S., Ker, D.F.E., Bise, R. and Kanade, T. (2011) “Tracking of Hematopoietic Stem Cells in Microscopy Images for Lineage Determination.” IEEE Transactions on Biomedical Engineering.
- Yin, Z., Ker, D.F.E. and Kanade, T. (2011) ‘Restoring DIC Microscopy Images from Multiple Shear Directions.’ (IPMI'11) Proceedings of the 22nd International Conference on Information Processing in Medical Imaging pg. 384-397.
- Yin, Z., Ker, D.F.E., Junkers, S.N., Kanade, T., Chen, M., Weiss, L.E. and Campbell, P.G. (2011) ‘Data-driven Prediction of Stem Cell Expansion Cultures.’ (EMBC’11) Proceedings of the 33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society pg. 3577 – 3580.
- Huh, S., Ker, D.F.E., Bise, R., Chen, M., and Kanade, T. (2011) ”Automated Mitosis Detection of Stem Cell Populations in Phase-Contrast Microscopy Images.” IEEE Transactions on Medical Imaging 30, 3, pg. 586-596.
By Chronological Order
2024 Journal Publication(s)
2023 Journal Publication(s)
2022 Journal Publication(s)
2021 Journal Publication(s)
2020 Journal Publication(s)
2019 Journal Publication(s)
2018 Journal Publication(s)
2017 Journal Publication(s)
2015 Journal Publication(s)
2014 Journal Publication(s)
2013 Journal Publication(s)
2012 Journal Publication(s)
2011 Journal Publication(s)
2024 Journal Publication(s)
- Zhang, X., Li, K., Wang, C.Y., Rao, Y., Tuan, R.S., Wang, D., Ker, D.F.E.¶ (Jul 2024) ‘Facile and Rapid Fabrication of a Novel 3D-Printable, Visible Light-Crosslinkable and Bioactive Polythiourethane for Large-to-Massive Rotator Cuff Tendon Repair’ Bioactive Materials 37, pg. 439. https://doi.org/10.1016/j.bioactmat.2024.03.036. ¶Corresponding author. Notable for developing a 3D-printable visible light-crosslinkable resin that can be prepared in 30 mins. This resin can generate mechanically strong and tendon-like biomaterial that withstands 10,000 cycles of loading without failure and can deliver pro-regenerative bioactive factors. This bioactive material biomechanically restored a large-to-massive (0.3-cm segmental defect with 1-cm gap) rabbit rotator cuff injury model to uninjured levels with at least 1-cm of tendon regeneration.
- Huang, S., Rao, Y., Zhou, M., Blocki, A.M., Ker, D.F.E., Tuan, R.S., Wang, D, (Jun 2024) ‘Engineering an extracellular matrix-functionalized, load-bearing tendon substitute for effective repair of large-to-massive tendon tears’ Bioactive Materials 36, pg. 221. https://doi.org/10.1016/j.bioactmat.2024.02.032. Notable for employing ex vivo, in vitro, and in vivo methods for fabricating a mechanically strong and bioactive material to address large tendon defects, which was assessed in two (rat and rabbit) rotator cuff injury models. and bioactive material to address large tendon defects, which was assessed in two (rat and rabbit) rotator cuff injury models.
- Wang, C.Y., Zhang, X., Wang, D, Yung, P.S.H., Tuan, R.S., Ker, D.F.E. (Mar 2024) ‘Optimized design of an enthesis-mimicking suture anchor-tendon hybrid graft for mechanically robust bone-tendon repair’ Acta Biomaterialia 176, pg. 277. https://doi.org/10.1016/j.actbio.2024.01.011 . *Notable for employing in silico, ex vivo, and in vivo methods to optimize design of bone-tendon biomaterials to address a grand challenge in musculoskeletal tissue engineering: Reducing stress concentrations to facilitate robust attachment of soft-to-hard tissues.
- Huang, S., Rao, Y., Ju, A.L., Ker, D.F.E., Blocki, A.M., Wang, D, Tuan, R.S. (Mar 2024) ‘Non-collagenous proteins, rather than the collagens, are key biochemical factors that mediate tenogenic bioactivity of tendon extracellular matrix’ Acta Biomaterialia 176, pg. 99. https://doi.org/10.1016/j.actbio.2023.12.032.
2023 Journal Publication(s)
- Kwan, K.Y.C., Ng, K.W.K., Rao, Y., Zhu, C., Qi, S., Tuan, R.S., Ker, D.F.E., Wang, D. (Oct 2023) ‘Biology of Tendon Aging: Mechanisms, Function, and Advanced Cell Therapeutics’ International Journal of Molecular Sciences 24, 20, pg. 15183 https://doi.org/10.3390/ijms242015183
- Li, K., Zhang, X., Wang, D., Ker, D.F.E. (Mar 2023) ‘Development of a Rabbit Chronic-Like Rotator Cuff Injury Model for Study of Fibrosis and Muscular Fatty Degeneration’ Journal of Visualized Experiments 146, pg. 213316. https://doi.org/10.3791/64828.
- Li, K., Zhang, X., Wang, D., Tuan, R.S., Ker, D.F.E. (Mar 2023) ‘Synergistic effects of growth factor-based serum-free medium and tendon-like substrate topography on tenogenesis of mesenchymal stem cells’ Biomaterials Advances 146, pg. 213316. https://doi.org/10.1016/j.bioadv.2023.213316. *Notable for developing a serum-free medium formulation that supports tenogenic proliferation and differentiation in vitro as well as demonstrating synergistic interactions between growth factor and substrate topography on mesenchymal stem cell tenogenesis in vitro.
2022 Journal Publication(s)
- Wang, D*., Zhang, X., Ng, K.W., Rao, Y., Wang, C.Y., Gharaibeh, B., Lin, S., Abrams, G., Safran, M., Cheung, E., Campbell, P., Weiss, L., Ker, D.F.E.* ¶, Yang, Y.P.¶ (Dec 2022) ‘Growth and Differentiation Factor-7 Immobilized, Mechanically Strong Quadrol-Hexamethylene Diisocyanate-Methacrylic Anhydride Polyurethane Polymer for Tendon Repair and Regeneration’ Acta Biomaterialia 154, pg. 108. *Co-first authors. ¶Co-corresponding authors. https://doi.org/10.1016/j.actbio.2022.10.029. *Notable for interfacing for methodology to augment synthetic biomaterials with bioactivity via growth factor immobilization for tendon regeneration.
- Shen, Y., Liu, H., Zhao, B., Pooh, H.L.E., Qi, S., Ker, D.F.E., Billiar, T.R., Cooper, G.M., Xu, Y., Wang, D. (Dec 2022) ‘Short-term Administration of the HIV Protease Inhibitor Saquinavir Improves Skull Bone Healing via Enhanced Osteoclastogenesis’ Plastic and Reconstructive Surgery 150, pg. 1264e. https://doi.org/10.1097/PRS.0000000000009734.
- Cheng, C.T., Wang, D., Wang, H., Ker, D.F.E. (Nov 2022) ‘Application of neural networks on in vitro-generated Raman spectra for label-free, ex vivo skeletal muscle detection’ Measurement 204, pg. 112172. https://doi.org/10.1016/j.measurement.2022.112172. *Notable for establishing similarity in complex Raman spectra between in vitro cultured cells and ex vivo tissue, and using of transfer learning for label-free detection of skeletal muscle.
- Zhang, X., Wang, D., Wang, Z., Ling, S.K., Yung, P.S., Tuan, R.S., Ker, D.F.E. (Sep 2022) ‘Clinical Perspectives for Repairing Rotator Cuff Injuries with Multi-Tissue Regenerative Approaches’ Journal of Orthopaedic Translation 36, pg. 91. https://www.sciencedirect.com/science/article/pii/S2214031X22000547?via%3Dihub.
- Rao, Y., Zhu, C., Suen, H. C., Huang, S., Liao, J., Ker, D.F.E., Tuan, R. S., and Wang, D. (Jul 2022) ‘Tenogenic induction of human adipose-derived stem cells by soluble tendon extracellular matrix: composition and transcriptomic analyses’ Stem Cell Research & Therapy 13, pg. 380. https://stemcellres.biomedcentral.com/articles/10.1186/s13287-022-03038-0.
2021 Journal Publication(s)
- Nishimura, K., Wang, C., Wantanabe, K., Ker, D.F.E., Bise, R. (Oct 2021) ‘Weakly Supervised Cell Instance Segmentation under Various Conditions’ Medical Image Analysis 73, pg. 102182. https://www.sciencedirect.com/science/article/pii/S1361841521002280.
- Zhang, X., Wang, D., Mak, K.K., Tuan, R.S., Ker, D.F.E. (Aug 2021) ‘Engineering Musculoskeletal Grafts for Multi-Tissue Unit Repair: Lessons from Developmental Biology and Wound Healing’ Frontiers in Physiology 12, pg. 1287. https://www.frontiersin.org/articles/10.3389/fphys.2021.691954/abstract.
- Wang, D., Zhang, X., Huang, S., Liu, Y., Fu, B.S., Mak, K.K., Blocki, A.M., Yung, P.S., Tuan, R.S., Ker, D.F.E. (May 2021) ‘Engineering Multi-Tissue Units for Regenerative Medicine: Bone-Tendon-Muscle Units of the Rotator Cuff’ Biomaterials 272, pg. 120789. https://www.sciencedirect.com/science/article/pii/S0142961221001459?via%3Dihub.
- Wang, D.*, Ker, D.F.E.*¶, Ng, K.W., Li, K., Gharaibeh, B., Safran, M., Cheung, E., Campbell, P.G., Weiss, L.E., Yang, Y.P.¶ (Mar 2021) ‘Combinatorial Mechanical-Gradation and Growth Factor-Biopatterning Strategy to Spatially Control Bone-Tendon-like Cell Differentiation and Tissue Formation’ NPG Asia Materials 13, pg. 26. *Co-first authors. ¶Co-corresponding authors. https://www.nature.com/articles/s41427-021-00294-z. *Notable for studying the interaction effect of biopatterned growth factors with substrate stiffness.
- Assunção, M., Yiu, C.H.K., Wan, H.Y., Wang, D., Ker, D.F.E., Tuan, R.S., Blocki, A. (Mar 2021) ‘Hyaluronic acid drives mesenchymal stromal cell-derived extracellular matrix assembly by promoting fibronectin fibrillogenesis’ Journal of Materials Chemistry Part B 9, pg. 7205. https://pubs.rsc.org/en/content/articlelanding/2021/TB/D1TB00268F#!divAbstract.
2020 Journal Publication(s)
- Shanjani, Y., Siebert, S.M., Ker, D.F.E., Mercado-Pagan, A.E., and Yang, Y.P. (Jun 2020) ‘Acoustic Patterning of Growth Factor for 3D Tissue Engineering’ Tissue Engineering Part A 26, 11-12, pg. 602. https://www.liebertpub.com/doi/full/10.1089/ten.TEA.2019.0271.
- Wang, D., Pun, C.C.M., Huang, S. Tang, T.C.M., Ho, K.K.W., Rothrauff, B.B., Yung, P.S.H., Blocki, A.M., Ker, D.F.E., and Tuan, R.S. (Apr 2020) ‘Tendon-derived Extracellular Matrix Induces Mesenchymal Stem Cell Tenogenesis via an Integrin/Transforming Growth Factor-β Cross-talk-Mediated Mechanism’ Federation of American Societies for Experimental Biology Journal 34, 6, pg. 8172. https://faseb.onlinelibrary.wiley.com/doi/abs/10.1096/fj.201902377RR.
- Wang, D., Gilbert, J.R., Zhang, X., Zhao, B., Ker, D.F.E., Cooper, G.M (Jan 2020) ‘Calvarial versus Long Bone Implications for Tailoring Skeletal Tissue Engineering’ Tissue Engineering Part B 26, 1, pg. 46. https://www.liebertpub.com/doi/full/10.1089/ten.TEB.2018.0353.
- Nishimura K., Hayashida J., Chenyang W., Ker, D.F.E., and Bise, R. (2020) “Weakly-Supervised Cell Tracking via Backward-and-Forward Propagation” (ECCV’20) 16th European Conference on Computer Vision (presented 23rd Aug 2020 - 28th Aug 2020, Glasgow, United Kingdom).
2019 Journal Publication(s)
- Ker, D.F.E. and Yang, Y.P. (Jun 2019) ‘Ruminants: Evolutionary Past and Future Impact’ Science (Perspective) 364, 6446, pg. 1130. https://science.sciencemag.org/content/364/6446/1130.
- Nishimura K., Ker, D.F.E., and Bise, R. (2019) “Cell Region Recognition in Microscopic Images Using Weakly Supervised Learning” (MIRU’19) 22nd Symposium on Image Recognition and Understanding (presented 29th Jul 2019 – 1st Aug 2019, Osaka, Japan).
- Nishimura K., Ker, D.F.E., and Bise, R. (2019) “Weakly Supervised Cell Segmentation in Dense By Propagating from Detection Map” (MICCAI’19) Medical Image Computing and Computer Assisted Intervention (presented 13th Oct 2019 – 17th Oct 2019, Shenzhen, China).
2018 Journal Publication(s)
- Ker, D.F.E., Eom, S., Sanami, S., Bise, R., Pascale, C., Yin, Z., Huh, S., Osuna-Highley, E., Junkers, S.N., Helfrich, C.J., Liang, P.W., Pan, J., Jeong, S., Kang, S.S., Liu, J., Nicholson, R., Sandbothe, M.F., Van, P.T., Liu, A., Chen, M., Kanade, T., Weiss, L.E. and Campbell P.G. (Nov 2018) ‘Phase Contrast Time-lapse Microscopy Datasets with Automated and Manual Cell Tracking Annotations’ Scientific Data 5, pg. 180237. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6233481/. *Notable for being the largest manually curated and computer-tracked phase contrast microscopy dataset of cells in the world.
- Ker, D.F.E., Wang, D., Sharma, S., Zhang, B., Passarelli, B., Neff, N., Li, C., Maloney, W., Quake S. and Yang, Y.P. (Oct 2018) ‘Identifying Deer Antler uhrf1 Proliferation and s100a10 Mineralization Genes Using Comparative RNA-seq’ Stem Cell Research & Therapy 9, 1, pg. 292. https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/30376879/. *Notable for development of a unique model to study rapid bone regeneration.
- Ker, D.F.E.*¶, Wang, D.*, Behn, A.W., Wang, E.T.H., Zhang, X., Zhou, B.Y., Mercado-Pagan, A.E., Kim, S., Kleimeyer, J., Gharaibeh, B., Shanjani, Y., Nelson, D., Safran, M., Cheung E., Campbell, P., Yang, Y.P.¶ (May 2018) ‘Functionally-Graded, Bone- and Tendon-Like Polyurethane for Rotator Cuff Repair’ Advanced Functional Materials 28, 20, pg. 1707107. *Co-first authors. ¶Co-corresponding authors. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5959293/. *Notable for development of a patented mechanically robust photocrosslinkable biomaterial that achieves bone- and tendon-like mechanical properties.
2017 Journal Publication(s)
- Shanjani, Y., Kang, Y., Zarnescu, L., Bowden, A.K., Koh, J., Ker, D.F.E. and Yang, Y.P. (Jan 2017) ‘Endothelial Pattern Formation in Hybrid Constructs of Additive Manufactured Porous Rigid Scaffolds and Cell-laden Hydrogels for Orthopedic Applications’ Journal of the Mechanical Behavior of Biomedical Materials 65, pg. 356. https://www.sciencedirect.com/science/article/pii/S175161611630306X.
2015 Journal Publication(s)
- Li, M., Li, H, Ker, D.F.E., Su, H. and Yin, Z. (Oct 2015) ‘Cell-Sensitive Phase Contrast Microscopy Imaging by Multiple Exposures’ Medical Image Analysis 25, 1, pg. 111. https://www.sciencedirect.com/science/article/pii/S1361841515000614.
- Ker, D.F.E., Wang, E.T.H., Sharma, R. and Yang, Y.P. (Sep 2015) ‘Development of mRuby2-transfected C3H10T1/2 Fibroblasts for Musculoskeletal Tissue Engineering’ PLoS ONE 10, 1, pg. e0139054. https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0139054. *A unique fluorescently labelled progenitor cell line that is available from American Type Culture Collection (https://www.atcc.org/products/crl-3268)
2014 Journal Publication(s)
- Mercado-Pagan, Ker, D.F.E., and Yang, Y. (Jun 2014) ‘Hemocompatibility Evaluation of Elastomeric Hollow Fiber Membranes as Vascular Grafts’ Journal of Biomaterials Applications 29, 4, pg. 557. https://journals.sagepub.com/doi/10.1177/0885328214537541.
- Li, H., Li, M., Ker, D.F.E., Su, H. and Yin, Z. (2014) “Cell-Sensitive Microscopy Imaging for Cell Image Segmentation” (MICCAI’14) Medical Image Computing and Computer Assisted Intervention 17(Pt 1), pg. 41-48.
2013 Journal Publication(s)
- Mercado-Pagan, A.E., Kang, Y., Ker, D.F.E., Park, S., Yao, J., Bishop, J., and Yang, Y. (Oct 2013) ‘Synthesis and Characterization of Novel Elastomeric Poly(D,L-Lactide Urethane) Maleate Composites for Bone Tissue Engineering’ European Polymer Journal 49, 10, pg. 3337. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4012890/.
2012 Journal Publication(s)
- Smith, D.M., Cray, J.J.Jr, Weiss, L.E., Ker, D.F.E., Shakir, S., Rottgers, S.A., Losee, J.E., Campbell, P.G. and Cooper, G.M. (Oct 2012) ‘Precise Control of Osteogenesis for Craniofacial Defect Repair: The Role of Direct Osteoprogenitor Contact in BMP-2-based Bioprinting’ Annals of Plastic Surgery 69, 4, pg. 485. https://journals.lww.com/annalsplasticsurgery/fulltext/2012/10000/Precise_Control_of_Osteogenesis_for_Craniofacial.38.aspx.
- Gao, Y., Smith, E., Ker, D.F.E., Campbell, P.G., Cheng, E., Zou, S., Lin, S., Wang, L., Halene, S. and Krause, D.S. (Mar 2012) ‘Role of RhoA Specific Guanine Exchange Factors in Regulation of Endomitosis in Megakaryocytes’ Developmental Cell 22, 3, pg. 573. https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22387001/.
- Huh, S., Ker, D.F.E., Su, H., Kanade, T. (2012) “Apoptosis Detection for Adherent Cell Populations in Time-Lapse Phase-Contrast Microscopy Images” (MICCAI'12) In Proc. International Conference on Medical Image Computing and Computer Assisted Intervention 15(Pt 1), pg.331-339.
- Huh, S., Eom, S., Ker, D.F.E., Weiss, L.E and Kanade, T. (2012) “Mitosis Detection of Hematopoietic Stem Cell Populations in Time-Lapse Phase-Contrast Microscope Images.” (ISBI'12) In Proceedings of IEEE International Symposium on Biomedical Imaging pg. 390-393.
2011 Journal Publication(s)
- Ker, D.F.E., Weiss, L.E., Junkers, S.N., Chen, M., Yin, Z., Sandboth, M.F., Huh, S., Eom, S., Bise, R., Osuna-Highley, E., Kanade, T. and Campbell, P.G. (Nov 2011) ‘An Engineered Approach to Stem Cell Culture: Automating the Decision Process for Real-Time Adaptive Subculture of Stem Cells’ PLoS ONE 6, 11, pg. e27672. https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0027672 *Notable for development of an online decision-making system that has similar performance as a skilled human operator for culturing cells.
- Ker, D.F.E., Nain, A., Weiss, L.E., Wang, J., Suhan, J., Amon, C. and Campbell, P.G. (Nov 2011) ‘Bioprinting of Growth Factors onto Aligned Sub-micron Fibrous Scaffolds for Simultaneous Control of Cell Differentiation and Alignment’ Biomaterials 32, 32, pg. 8097. https://www.sciencedirect.com/science/article/pii/S0142961211007964. *Notable for being the first demonstration of patterning a primitive muscle-tendon-bone unit on polyurethane nanofibers.
- Ker, D.F.E., Chu, B., Phillippi, J.A., Gharaibeh, B., Huard, J., Weiss, L.E., Campbell, P.G. (May 2011) ‘Engineering Spatial Control of Multiple Differentiation Fates Within a Stem Cell Population’ Biomaterials 32, 13, pg. 3413. https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21316755/. *Notable for being the first demonstration of patterning a primitive muscle-tendon-bone unit.
- Eom, S., Huh, S., Ker, D.F.E., Bise, R. and Kanade, T. (2011) “Tracking of Hematopoietic Stem Cells in Microscopy Images for Lineage Determination.” IEEE Transactions on Biomedical Engineering.
- Yin, Z., Ker, D.F.E. and Kanade, T. (2011) ‘Restoring DIC Microscopy Images from Multiple Shear Directions.’ (IPMI'11) Proceedings of the 22nd International Conference on Information Processing in Medical Imaging pg. 384-397.
- Yin, Z., Ker, D.F.E., Junkers, S.N., Kanade, T., Chen, M., Weiss, L.E. and Campbell, P.G. (2011) ‘Data-driven Prediction of Stem Cell Expansion Cultures.’ (EMBC’11) Proceedings of the 33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society pg. 3577 – 3580.
- Huh, S., Ker, D.F.E., Bise, R., Chen, M., and Kanade, T. (2011) ”Automated Mitosis Detection of Stem Cell Populations in Phase-Contrast Microscopy Images.” IEEE Transactions on Medical Imaging 30, 3, pg. 586-596.